Hotspot

chlB

Genome · 90855-94329 · 3475 bp

Back to Corallina

Pi 0.035

MarkerSeek score 62.70

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 3475
variable_sites: 180
pi: 0.035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	TGGAACAGGGAATCATTCAGT	GGTAAAAGATTGCCCGCATC	42.9	50.0	56.9	57.5	1060-1069 bp	0.666667	49.0
chlB_p2	TGGAACAGGGAATCATTCAGTT	GGTAAAAGATTGCCCGCATC	40.9	50.0	57.6	57.5	1060-1069 bp	0.666667	46.7
chlB_p3	TGGAACAGGGAATCATTCAGT	AGGTAAAAGATTGCCCGCAT	42.9	45.0	56.9	57.9	1061 bp	0.333333	35.4
chlB_p4	TGGAACAGGGAATCATTCAGTT	AGGTAAAAGATTGCCCGCAT	40.9	45.0	57.6	57.9	1061 bp	0.333333	33.1
chlB_p5	TGAGTAGAGGTTTGTCTCGA	AGGTAAAAGATTGCCCGCAT	45.0	45.0	55.3	57.9	908 bp	0.333333	30.3