Hotspot

argB

Genome · 56672-57523 · 852 bp

Back to Corallina

Pi 0.0246

MarkerSeek score 60.50

Primer available yes

Label argB

Feature Metadata

label_name: argB
feature_type: gene
region: Genome
length_bp: 852
variable_sites: 31
pi: 0.0246
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
argB_p1	TAGCACCACCAGGATAACCA	CAACCACTCAGCCATCTCTC	50.0	55.0	58.0	58.0	1824-1834 bp	1.0	84.3
argB_p2	GCACCACCAGGATAACCAAA	CAACCACTCAGCCATCTCTC	50.0	55.0	58.1	58.0	1822-1832 bp	1.0	84.0
argB_p3	TAGCACCACCAGGATAACCA	CGACCTTGGGCTTATGAGTC	50.0	55.0	58.0	58.1	1732-1738 bp	1.0	83.8
argB_p4	GCACCACCAGGATAACCAAA	CGACCTTGGGCTTATGAGTC	50.0	55.0	58.1	58.1	1730-1736 bp	1.0	83.5
argB_p5	TAGCACCACCAGGATAACCA	TTGAACTACGGTCCCTAGCA	50.0	50.0	58.0	58.1	1977 bp	0.333333	52.4