Hotspot

nblA-cpeB

Genome · 152222-153144 · 923 bp

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Pi 0.103

MarkerSeek score 81.90

Primer available yes

Label nblA-cpeB

Feature Metadata

label_name: nblA-cpeB
feature_type: igs
region: Genome
length_bp: 923
variable_sites: 95
pi: 0.103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
nblA-cpeB_p1	TTTTATACAAAACTCAAACCTGT	CTCGGGAAAATGCATCAAGC	26.1	50.0	52.8	57.8	963-1027 bp	1.0	51.7
nblA-cpeB_p2	ATTTTATACAAAACTCAAACCTGT	CTCGGGAAAATGCATCAAGC	25.0	50.0	53.4	57.8	964-1028 bp	1.0	51.7
nblA-cpeB_p3	TTTATACAAAACTCAAACCTGTAA	CTCGGGAAAATGCATCAAGC	25.0	50.0	52.8	57.8	962-1026 bp	1.0	51.7
nblA-cpeB_p4	TTTTATACAAAACTCAAACCTGTA	CTCGGGAAAATGCATCAAGC	25.0	50.0	52.8	57.8	963-1027 bp	1.0	51.7
nblA-cpeB_p5	TTTTATACAAAACTCAAACCTGT	ACTACAACTCGGGAAAATGCA	26.1	42.9	52.8	57.9	970-1034 bp	1.0	51.6