Hotspot

trnG2(tcc)-ycf4

Genome · 138430-139928 · 1499 bp

Back to Corallina

Pi 0.107

MarkerSeek score 56.90

Primer available yes

Label trnG2(tcc)-ycf4

Feature Metadata

label_name: trnG2(tcc)-ycf4
feature_type: igs
region: Genome
length_bp: 1499
variable_sites: 23
pi: 0.107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.10
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG2(tcc)-ycf4_p1	GGTTTTCATTCGCCACATGG	GGAACGTAGGTGCTGGTTAC	50.0	55.0	58.0	58.3	906-2253 bp	1.0	85.3
trnG2(tcc)-ycf4_p2	GGTTTTCATTCGCCACATGG	TATGGAACGTAGGTGCTGGT	50.0	50.0	58.0	58.4	909-2256 bp	1.0	84.1
trnG2(tcc)-ycf4_p3	GGTTTTCATTCGCCACATGG	GAACGTAGGTGCTGGTTACA	50.0	50.0	58.0	57.6	905-2252 bp	1.0	84.0
trnG2(tcc)-ycf4_p4	GGTTTTCATTCGCCACATGG	ATTCCTTTGACGCGTGTAGG	50.0	50.0	58.0	58.3	709-2056 bp	0.666667	69.7
trnG2(tcc)-ycf4_p5	ACTCATAATCCGCCATTCCG	ATTCCTTTGACGCGTGTAGG	50.0	50.0	57.8	58.3	427-1774 bp	0.666667	68.2