Hotspot

rps5-rpl18

Genome · 71769-71786 · 18 bp

Back to Corallina

Pi 0.148
MarkerSeek score 76.00
Primer available yes
Label rps5-rpl18

Feature Metadata

label_name
rps5-rpl18
feature_type
igs
region
Genome
length_bp
18
variable_sites
4
pi
0.148
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps5-rpl18_p1 TGCTTCTTGTTCTCTTTGTT GCAGATGCAACGAGAAATGA 35.0 45.0 53.2 56.8 99 bp 1.0 43.2
rps5-rpl18_p2 TTGCTTCTTGTTCTCTTTGT GCAGATGCAACGAGAAATGA 35.0 45.0 53.2 56.8 100 bp 1.0 43.2
rps5-rpl18_p3 TGCTTCTTGTTCTCTTTGTTT GCAGATGCAACGAGAAATGA 33.3 45.0 54.1 56.8 99 bp 1.0 43.2
rps5-rpl18_p4 TTGCTTCTTGTTCTCTTTGTT GCAGATGCAACGAGAAATGA 33.3 45.0 54.1 56.8 100 bp 1.0 43.2
rps5-rpl18_p5 TTTGCTTCTTGTTCTCTTTGT GCAGATGCAACGAGAAATGA 33.3 45.0 54.1 56.8 101 bp 1.0 43.2