Hotspot

dnaB-trnF(gaa)

Genome · 49795-50638 · 844 bp

Back to Corallina

Pi 0.0434
MarkerSeek score 71.70
Primer available yes
Label dnaB-trnF(gaa)

Feature Metadata

label_name
dnaB-trnF(gaa)
feature_type
igs
region
Genome
length_bp
844
variable_sites
55
pi
0.0434
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaB-trnF(gaa)_p1 ATTGCAGGTAGACCCTCCAT GGTGCAAGACCTCTTCGTAG 50.0 55.0 58.1 58.0 1482 bp 0.333333 52.5
dnaB-trnF(gaa)_p2 ATTGCAGGTAGACCCTCCAT TCAGTCCACTGCTCTACCAA 50.0 50.0 58.1 58.0 1206 bp 0.333333 52.4
dnaB-trnF(gaa)_p3 ATTGCAGGTAGACCCTCCAT TCCACTGCTCTACCAACTGA 50.0 50.0 58.1 58.0 1202 bp 0.333333 52.4
dnaB-trnF(gaa)_p4 ATTGCAGGTAGACCCTCCAT TTCAGTCCACTGCTCTACCA 50.0 50.0 58.1 58.0 1207 bp 0.333333 52.4
dnaB-trnF(gaa)_p5 ATTGCAGGTAGACCCTCCAT AGTCCACTGCTCTACCAACT 50.0 50.0 58.1 58.0 1204 bp 0.333333 52.4