Hotspot

trnL(tag)-psbA

Genome · 4987-5133 · 147 bp

Back to Corallina

Pi 0.0499

MarkerSeek score 76.20

Primer available yes

Label trnL(tag)-psbA

Feature Metadata

label_name: trnL(tag)-psbA
feature_type: igs
region: Genome
length_bp: 147
variable_sites: 11
pi: 0.0499
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(tag)-psbA_p1	AGAGTTCGAGTCTCTCCGTC	TACAGAAACGTTCCCACAGG	55.0	50.0	58.3	57.5	224-226 bp	1.0	78.2
trnL(tag)-psbA_p2	GAGAGTTCGAGTCTCTCCGT	TACAGAAACGTTCCCACAGG	55.0	50.0	58.3	57.5	225-227 bp	1.0	78.1
trnL(tag)-psbA_p3	TAAGCGGACGTGGTGAAATT	TACAGAAACGTTCCCACAGG	45.0	50.0	57.8	57.5	286-288 bp	1.0	77.9
trnL(tag)-psbA_p4	CGGACGTGGTGAAATTGGTA	TACAGAAACGTTCCCACAGG	50.0	50.0	58.2	57.5	282-284 bp	1.0	77.8
trnL(tag)-psbA_p5	TAAGCGGACGTGGTGAAATT	AGTACAGAAACGTTCCCACAG	45.0	47.6	57.8	57.9	288-290 bp	1.0	73.4