Hotspot

rpl2

IRa · 153796-155294 · 1499 bp

Back to Coptis

Pi 0.00317
MarkerSeek score 31.00
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRa
length_bp
1499
variable_sites
7
pi
0.00317
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.73
markerseek_score
31.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 GAATGGTTAAAGCGCCCAAC TTTCTGCCCTCATGTTGAGC 50.0 50.0 58.0 58.5 2161-2166 bp 0.25 46.7
rpl2_p2 AGCATTGGGTCGAACTCTTC TTTCTGCCCTCATGTTGAGC 50.0 50.0 57.9 58.5 1828-1833 bp 0.25 46.1
rpl2_p3 GCATTGGGTCGAACTCTTCT TTTCTGCCCTCATGTTGAGC 50.0 50.0 57.9 58.5 1827-1832 bp 0.25 46.1
rpl2_p4 TCCCACGGAATCGAATTTGG TTTCTGCCCTCATGTTGAGC 50.0 50.0 58.3 58.5 2367-2372 bp 0.25 44.8
rpl2_p5 CGCTTCAACCGGGTTATTCT TTTCTGCCCTCATGTTGAGC 50.0 50.0 58.3 58.5 1702-1707 bp 0.25 44.8