Hotspot

ndhD

SSC · 116362-117891 · 1530 bp

Pi 0.00686

MarkerSeek score 50.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 36
pi: 0.00686
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TAATGAGGCATGGGGATCCT	GCATGAGACAACTCGTAGCA	50.0	50.0	57.9	58.0	2163-2191 bp	1.0	83.2
ndhD_p2	GCTAATGAGGCATGGGGATC	GCATGAGACAACTCGTAGCA	55.0	50.0	58.2	58.0	2165-2193 bp	1.0	82.9
ndhD_p3	TCGTGTCATTAGTCTCGGGT	GCATGAGACAACTCGTAGCA	50.0	50.0	58.2	58.0	2226-2254 bp	1.0	82.8
ndhD_p4	AATGAGGCATGGGGATCCTA	GCATGAGACAACTCGTAGCA	50.0	50.0	57.9	58.0	2162-2190 bp	0.875	77.9
ndhD_p5	TGAGGCATGGGGATCCTATT	GCATGAGACAACTCGTAGCA	50.0	50.0	57.9	58.0	2160-2188 bp	0.875	77.9