Hotspot

rpl23-rpl2

IRa · 153790-153795 · 6 bp

Back to Coptis

Pi 0.0417
MarkerSeek score 67.30
Primer available yes
Label rpl23-rpl2

Feature Metadata

label_name
rpl23-rpl2
feature_type
igs
region
IRa
length_bp
6
variable_sites
1
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-rpl2_p1 CGCTTCAACCGGGTTATTCT TGCTCGGGGTAGAAGTTTTG 50.0 50.0 58.3 57.8 105 bp 1.0 77.2
rpl23-rpl2_p2 CGCTTCAACCGGGTTATTCT GCTCGGGGTAGAAGTTTTGT 50.0 50.0 58.3 57.8 104 bp 1.0 77.2
rpl23-rpl2_p3 CGCTTCAACCGGGTTATTCT GTGCTCGGGGTAGAAGTTTT 50.0 50.0 58.3 57.8 106 bp 1.0 77.2
rpl23-rpl2_p4 CGCTTCAACCGGGTTATTCT GGATTTCACTTGACTGCCGA 50.0 50.0 58.3 58.2 140 bp 1.0 76.9
rpl23-rpl2_p5 ATCATTACGCTTCAACCGGG TGCTCGGGGTAGAAGTTTTG 50.0 50.0 58.3 57.8 112 bp 1.0 76.6