Hotspot

trnL-UAG-ccsA

SSC · 114989-115102 · 114 bp

Back to Coptis

Pi 0.0103
MarkerSeek score 60.90
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
114
variable_sites
4
pi
0.0103
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT ACAGCCCTATGATTTCGTAGAC 50.0 45.5 58.0 57.7 292-297 bp 1.0 63.8
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG ACAGCCCTATGATTTCGTAGAC 50.0 45.5 58.0 57.7 291-296 bp 1.0 63.8
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA ACAGCCCTATGATTTCGTAGAC 50.0 45.5 57.9 57.7 282-287 bp 1.0 62.9
trnL-UAG-ccsA_p4 GTAGACACGCTGCTCTTAGG ACAGCCCTATGATTTCGTAGAC 55.0 45.5 57.8 57.7 283-288 bp 1.0 62.4
trnL-UAG-ccsA_p5 GGTAGACACGCTGCTCTTAG ACAGCCCTATGATTTCGTAGAC 55.0 45.5 57.8 57.7 284-289 bp 1.0 62.3