Hotspot

rpl32-trnL-UAG

SSC · 114307-114908 · 602 bp

Back to Coptis

Pi 0.0179
MarkerSeek score 64.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
602
variable_sites
33
pi
0.0179
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGGCGATAAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 405-737 bp 1.0 88.4
rpl32-trnL-UAG_p2 GGCGGCGATAAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 406-738 bp 1.0 88.4
rpl32-trnL-UAG_p3 GGCGGCGATAAAAGCTTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 415-747 bp 1.0 87.4
rpl32-trnL-UAG_p4 GGCGGCGATAAAAGCTTTTT AACGATCCACACAGAAGAGC 45.0 50.0 58.0 57.9 1030-1365 bp 1.0 86.5
rpl32-trnL-UAG_p5 TTTCCCGTCACTTCAGTTCG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 733-1317 bp 0.875 81.4