Hotspot

infA-rps8

LSC · 80418-80538 · 121 bp

Back to Coptis

Pi 0.0304
MarkerSeek score 64.80
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
LSC
length_bp
121
variable_sites
14
pi
0.0304
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 ATCGTCTGGCAGTATCCGTA GAACTCGAGTTTTTGGTGCG 50.0 50.0 58.0 58.0 949-957 bp 1.0 83.2
infA-rps8_p2 TCGTCTGGCAGTATCCGTAT GAACTCGAGTTTTTGGTGCG 50.0 50.0 58.0 58.0 948-956 bp 1.0 83.2
infA-rps8_p3 ATCGTCTGGCAGTATCCGTA AAAAACTCGGAGGCACCAAT 50.0 45.0 58.0 58.0 703-704 bp 0.875 77.7
infA-rps8_p4 TATCGTCTGGCAGTATCCGT AAAAACTCGGAGGCACCAAT 50.0 45.0 58.0 58.0 704-705 bp 0.875 77.7
infA-rps8_p5 TCGTCTGGCAGTATCCGTAT AAAAACTCGGAGGCACCAAT 50.0 45.0 58.0 58.0 702-703 bp 0.875 77.7