Hotspot

petA-psbJ

LSC · 63747-64502 · 756 bp

Back to Coptis

Pi 0.0141
MarkerSeek score 70.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
756
variable_sites
40
pi
0.0141
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGTAATAGGGGAAGGGGTCA GCTGGTATTCTTGTGATCGGT 55.0 47.6 57.8 58.1 863-879 bp 1.0 76.0
petA-psbJ_p2 ATCCTATATACGTGGGCGGT GCTGGTATTCTTGTGATCGGT 50.0 47.6 57.8 58.1 880-896 bp 1.0 75.7
petA-psbJ_p3 GGTAATAGGGGAAGGGGTCA AGCTGGTATTCTTGTGATCGG 55.0 47.6 57.8 57.8 864-880 bp 1.0 75.2
petA-psbJ_p4 ATCCTATATACGTGGGCGGT AGCTGGTATTCTTGTGATCGG 50.0 47.6 57.8 57.8 881-897 bp 1.0 74.9
petA-psbJ_p5 CTATTTTGTCTCCGGACCCC GCTGGTATTCTTGTGATCGGT 55.0 47.6 57.7 58.1 931-947 bp 1.0 74.5