Hotspot

ndhC-trnV-UAC

LSC · 51545-52715 · 1171 bp

Back to Coptis

Pi 0.0191
MarkerSeek score 67.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1171
variable_sites
74
pi
0.0191
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.1 57.8 1067-1242 bp 1.0 56.3
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.1 57.5 1066-1241 bp 1.0 53.6
ndhC-trnV-UAC_p3 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 1055-1230 bp 1.0 52.5
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.6 57.8 1066-1241 bp 1.0 52.3
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.6 57.8 1067-1242 bp 1.0 52.3