Hotspot

psbZ-trnG-GCC

LSC · 36846-37221 · 376 bp

Back to Coptis

Pi 0.0466
MarkerSeek score 91.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
376
variable_sites
43
pi
0.0466
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
91.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TGCTTCTTCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.0 57.8 532-547 bp 1.0 80.8
psbZ-trnG-GCC_p2 GCTTCTTCTGATGGTTGGTCA CGTCTTCTCCTTGGCAAAGA 47.6 50.0 58.2 57.8 531-546 bp 1.0 78.7
psbZ-trnG-GCC_p3 TGCTTCTTCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 47.6 50.0 58.2 57.8 532-547 bp 1.0 78.7
psbZ-trnG-GCC_p4 TTGCTTCTTCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 42.9 50.0 57.8 57.8 533-548 bp 1.0 78.6
psbZ-trnG-GCC_p5 TGCTTCTTCTGATGGTTGGT TACCATTCGACCATATCCGC 45.0 50.0 57.0 57.3 505-520 bp 1.0 76.8