Hotspot

petN-psbM

LSC · 29011-30206 · 1196 bp

Back to Coptis

Pi 0.00902
MarkerSeek score 64.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1196
variable_sites
36
pi
0.00902
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA ACGTAAAAACGGTCAGCCAA 45.0 45.0 58.0 58.3 1054-1301 bp 1.0 83.6
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG ACGTAAAAACGGTCAGCCAA 50.0 45.0 57.8 58.3 1060-1307 bp 1.0 82.1
petN-psbM_p3 TCTTGCTTGGGCTGCTTTAA TACGTAAAAACGGTCAGCCA 45.0 45.0 58.0 57.5 1055-1302 bp 1.0 82.1
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG TACGTAAAAACGGTCAGCCA 50.0 45.0 57.8 57.5 1061-1308 bp 1.0 80.6
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA CGTAAAAACGGTCAGCCAAA 45.0 45.0 58.0 57.2 1053-1300 bp 1.0 80.2