Hotspot

rpl22

LSC · 85995-86321 · 327 bp

Back to Copernicia

Pi 0

MarkerSeek score 20.40

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 327
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GCGAACCAAAAGGAACGATG	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	702-706 bp	1.0	82.0
rpl22_p2	GCGAACCAAAAGGAACGATG	TAGTAACTTGGTCTCGGGCA	50.0	50.0	58.0	58.1	841-845 bp	1.0	81.1
rpl22_p3	GCGAACCAAAAGGAACGATG	GATCCACTTGGCTACATCCG	50.0	55.0	58.0	58.1	1088-1092 bp	1.0	80.3
rpl22_p4	GCGAACCAAAAGGAACGATG	CAATCCACTGCCTTGATCCA	50.0	50.0	58.0	57.9	1102-1106 bp	1.0	80.2
rpl22_p5	GCGAACCAAAAGGAACGATG	AATCCACTGCCTTGATCCAC	50.0	50.0	58.0	57.9	1101-1105 bp	1.0	80.2