Hotspot

accD

LSC · 59851-61332 · 1482 bp

Back to Copernicia

Pi 0.0103

MarkerSeek score 50.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 23
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GGATTGAGCCGAACCGAATA	GGTCCCATCGGATCTAGACA	50.0	55.0	57.8	58.0	2522-2584 bp	1.0	82.2
accD_p2	GGATTGAGCCGAACCGAATA	TGGTCCCATCGGATCTAGAC	50.0	55.0	57.8	58.0	2523-2585 bp	1.0	82.2
accD_p3	GGATTGAGCCGAACCGAATA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	2468-2530 bp	1.0	82.1
accD_p4	GGATTGAGCCGAACCGAATA	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	2474-2536 bp	1.0	81.5
accD_p5	GGATTGAGCCGAACCGAATA	GTCATAGAATGGGTGCCTCG	50.0	55.0	57.8	58.1	2403-2465 bp	1.0	81.4