Hotspot

ndhF-rpl32

SSC · 115259-115535 · 277 bp

Back to Copernicia

Pi 0.0385

MarkerSeek score 68.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 277
variable_sites: 16
pi: 0.0385
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGAAAAGGTATTATCCATGCA	TCCCTTTTTCTTCCAAATATTCCT	37.5	33.3	57.0	56.2	393-452 bp	1.0	43.5
ndhF-rpl32_p2	TGGAAGAAAAGGTATTATCCATGC	TCCCTTTTTCTTCCAAATATTCCT	37.5	33.3	57.0	56.2	394-453 bp	1.0	43.4
ndhF-rpl32_p3	GTGGAAGAAAAGGTATTATCCATGC	TCCCTTTTTCTTCCAAATATTCCT	40.0	33.3	58.3	56.2	395-454 bp	1.0	43.4
ndhF-rpl32_p4	CTGTAAGTGGAAGAAAAGGT	TCCCTTTTTCTTCCAAATATTCCT	40.0	33.3	52.7	56.2	401-460 bp	1.0	43.4
ndhF-rpl32_p5	GGAAGAAAAGGTATTATCCATGCA	GCAGTTAAATATCCCTTTTTCTTCCA	37.5	34.6	57.0	58.2	404-463 bp	1.0	43.4