Hotspot

rpl22-rps19

IRb · 86322-86524 · 203 bp

Back to Copernicia

Pi 0.069

MarkerSeek score 87.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 203
variable_sites: 21
pi: 0.069
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	50.0	50.0	58.5	58.0	299-303 bp	1.0	81.4
rpl22-rps19_p2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	50.0	55.0	58.5	57.8	304-308 bp	1.0	80.0
rpl22-rps19_p3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	50.0	50.0	58.5	57.7	300-304 bp	1.0	79.3
rpl22-rps19_p4	CATAGGAACGTCCGCGAATC	TCTGACTTTCGCGAGACATG	55.0	50.0	58.9	58.0	307-311 bp	1.0	78.3
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	50.0	50.0	58.5	58.5	323-327 bp	1.0	77.5