Hotspot

accD-psaI

LSC · 61333-61617 · 285 bp

Back to Copernicia

Pi 0.0537

MarkerSeek score 71.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 285
variable_sites: 20
pi: 0.0537
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGTACCCGATGGTTCAC	GGTCCCATCGGATCTAGACA	55.0	55.0	58.0	58.0	498-560 bp	1.0	87.5
accD-psaI_p2	GACAGTACCCGATGGTTCAC	TGGTCCCATCGGATCTAGAC	55.0	55.0	58.0	58.0	499-561 bp	1.0	87.5
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	387-449 bp	0.666667	71.0
accD-psaI_p4	CCACGGTTTCTTTCCCTTGA	TGGTCCCATCGGATCTAGAC	50.0	55.0	58.0	58.0	388-450 bp	0.666667	71.0
accD-psaI_p5	TCCACGGTTTCTTTCCCTTG	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	388-450 bp	0.666667	71.0