Hotspot

rpoB-trnC-GCA

LSC · 27404-28579 · 1176 bp

Back to Copernicia

Pi 0.0051

MarkerSeek score 62.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 9
pi: 0.0051
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1244-1263 bp	1.0	73.1
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1245-1264 bp	1.0	72.9
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.4	58.8	1281-1300 bp	1.0	71.2
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TTTGTTGATCAGGCGACACC	50.0	50.0	57.6	58.8	1282-1301 bp	1.0	71.0
rpoB-trnC-GCA_p5	TCCCTCATTTCCATTCCGGA	GACACCCGGATTTGAACTGG	50.0	55.0	58.4	58.8	1267-1286 bp	1.0	70.6