Hotspot

matK-trnK-UUU

LSC · 3276-4150 · 875 bp

Back to Copernicia

Pi 0.0122

MarkerSeek score 71.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 14
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	55.9	58.2	962-978 bp	1.0	66.5
matK-trnK-UUU_p2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	55.9	58.2	961-977 bp	1.0	66.5
matK-trnK-UUU_p3	ACTCCTTAAAGAGAAGCGGA	GTCGTGCAGTGCAATTCAAT	45.0	45.0	55.9	57.7	1052-1068 bp	1.0	64.8
matK-trnK-UUU_p4	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	55.9	57.5	975-991 bp	1.0	64.1
matK-trnK-UUU_p5	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	45.0	50.0	55.9	58.5	978-994 bp	1.0	63.8