Hotspot

petB

LSC · 76307-77703 · 1397 bp

Back to Convallaria

Pi 0.00239

MarkerSeek score 60.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 5
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCGTTCTGGTAGTTCGACTG	ATTGGTCCTCCCCGATATGA	55.0	50.0	58.0	57.6	1525-1527 bp	1.0	76.8
petB_p2	CCGTTCTGGTAGTTCGACTG	TTGGTCCTCCCCGATATGAT	55.0	50.0	58.0	57.6	1524-1526 bp	1.0	76.8
petB_p3	TCTGGTAGTTCGACTGTGGA	ATTGGTCCTCCCCGATATGA	50.0	50.0	57.7	57.6	1521-1523 bp	1.0	74.7
petB_p4	TCTGGTAGTTCGACTGTGGA	TTGGTCCTCCCCGATATGAT	50.0	50.0	57.7	57.6	1520-1522 bp	1.0	74.7
petB_p5	CCGTTCTGGTAGTTCGACTG	CGATTGGTCCTCCCCGATAT	55.0	55.0	58.0	58.7	1527-1529 bp	1.0	74.3