Hotspot

ccsA-ndhD

SSC · 119872-120078 · 207 bp

Back to Convallaria

Pi 0.0386
MarkerSeek score 77.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
207
variable_sites
12
pi
0.0386
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGATCA TCAGTTGACAAGGTACAAGCT 36.4 42.9 55.0 57.2 357 bp 1.0 42.7
ccsA-ndhD_p2 TGGATATGCTATTTTGGGATCA TCGCTATCAGTTGACAAGGT 36.4 45.0 55.0 56.6 363 bp 1.0 42.6
ccsA-ndhD_p3 TGGATATGCTATTTTGGGATCAA TCGCTATCAGTTGACAAGGT 34.8 45.0 55.7 56.6 363 bp 1.0 42.6
ccsA-ndhD_p4 TTGGATATGCTATTTTGGGATCA TCGCTATCAGTTGACAAGGT 34.8 45.0 55.7 56.6 364 bp 1.0 42.6
ccsA-ndhD_p5 TGGATATGCTATTTTGGGATCA TTCGCTATCAGTTGACAAGGT 36.4 42.9 55.0 57.3 364 bp 1.0 42.6