Hotspot

ndhF-rpl32

SSC · 116661-117606 · 946 bp

Back to Convallaria

Pi 0.00565

MarkerSeek score 53.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 946
variable_sites: 8
pi: 0.00565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTGGAAATGGAAGAAAGGGT	ACTGACTTTGCTAAAGAAAAAGCT	42.9	33.3	57.1	57.5	1112-1127 bp	1.0	40.7
ndhF-rpl32_p2	AACTGGAAATGGAAGAAAGGGT	ACTGACTTTGCTAAAGAAAAAGCT	40.9	33.3	57.8	57.5	1113-1128 bp	1.0	40.7
ndhF-rpl32_p3	ACTGGAAATGGAAGAAAGGGT	ACTGACTTTGCTAAAGAAAAAGC	42.9	34.8	57.1	56.2	1112-1127 bp	1.0	40.7
ndhF-rpl32_p4	ACTGGAAATGGAAGAAAGGGT	CTGACTTTGCTAAAGAAAAAGCT	42.9	34.8	57.1	55.9	1111-1126 bp	1.0	40.7
ndhF-rpl32_p5	AACTGGAAATGGAAGAAAGGGT	ACTGACTTTGCTAAAGAAAAAGC	40.9	34.8	57.8	56.2	1113-1128 bp	1.0	40.7