Hotspot

atpB-rbcL

LSC · 55590-56376 · 787 bp

Back to Convallaria

Pi 0.00254

MarkerSeek score 60.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 3
pi: 0.00254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	894-895 bp	1.0	68.3
atpB-rbcL_p2	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.5	56.6	895-896 bp	1.0	65.4
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	57.9	57.2	894-895 bp	1.0	64.5
atpB-rbcL_p4	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	918-919 bp	1.0	64.0
atpB-rbcL_p5	TTCGTCAAGTGTGGAAACCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	858-859 bp	1.0	63.7