Hotspot

ndhC-trnV-UAC

LSC · 51162-52654 · 1493 bp

Back to Convallaria

Pi 0.00223
MarkerSeek score 60.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1493
variable_sites
5
pi
0.00223
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGTCCTTCACTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.8 57.8 1638-1647 bp 1.0 76.9
ndhC-trnV-UAC_p2 GGTCCTTCACTAATCGGAGC AGCCCAATGCAACAAAATGC 55.0 45.0 57.8 58.5 1720-1729 bp 1.0 74.7
ndhC-trnV-UAC_p3 GGTCCTTCACTAATCGGAGC GAAGGTCTACGGTTCGAGTC 55.0 55.0 57.8 57.5 1637-1646 bp 1.0 74.2
ndhC-trnV-UAC_p4 GGTCCTTCACTAATCGGAGC GTTCGAGTCCGTATAGCCCT 55.0 55.0 57.8 58.7 1626-1635 bp 1.0 73.0
ndhC-trnV-UAC_p5 GGTCCTTCACTAATCGGAGC GAAAGAGCCCAATGCAACAA 55.0 45.0 57.8 57.2 1725-1734 bp 1.0 71.9