Hotspot

rps16-trnQ-UUG

LSC · 5842-7153 · 1312 bp

Back to Convallaria

Pi 0.00457

MarkerSeek score 53.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1312
variable_sites: 9
pi: 0.00457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGCTTTCTACCACAGCGTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1374-1376 bp	1.0	79.6
rps16-trnQ-UUG_p2	TTGCTTTCTACCACAGCGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1375-1377 bp	1.0	79.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1385-1387 bp	1.0	78.8
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1396-1398 bp	1.0	78.3
rps16-trnQ-UUG_p5	GCTTTCTACCACAGCGTTTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	1373-1375 bp	1.0	75.1