Hotspot

ycf90

Genome · 58790-60211 · 1422 bp

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Pi 0.212

MarkerSeek score 79.70

Primer available yes

Label ycf90

Feature Metadata

label_name: ycf90
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 302
pi: 0.212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf90_p1	TTTGGTTTGGTATCGGTGCA	TTTAGTAGGTGGTTTCGCGG	45.0	50.0	58.0	57.9	1869 bp	0.5	59.2
ycf90_p2	TTTGGTTTGGTATCGGTGCA	ATGGCAAACACTGGTCGAAT	45.0	45.0	58.0	58.1	1907 bp	0.5	59.1
ycf90_p3	GGGTTAGCAGTAATGGCTCC	TTTAGTAGGTGGTTTCGCGG	55.0	50.0	58.0	57.9	2134 bp	0.5	59.1
ycf90_p4	GGGTTAGCAGTAATGGCTCC	ATGGCAAACACTGGTCGAAT	55.0	45.0	58.0	58.1	2172 bp	0.5	59.0
ycf90_p5	GGCTCCTACTCAAATGGGTG	TTTAGTAGGTGGTTTCGCGG	55.0	50.0	58.0	57.9	2120 bp	0.5	59.0