Hotspot

ycf39

Genome · 63443-64432 · 990 bp

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Pi 0.166

MarkerSeek score 79.30

Primer available yes

Label ycf39

Feature Metadata

label_name: ycf39
feature_type: gene
region: Genome
length_bp: 990
variable_sites: 163
pi: 0.166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf39_p1	AATTTTCGATAGCCGGGGTC	CAAGGTCACGACGATTAGGA	50.0	50.0	58.0	57.1	1676 bp	0.5	52.2
ycf39_p2	AATTTTCGATAGCCGGGGTC	TCAAGGTCACGACGATTAGG	50.0	50.0	58.0	57.1	1677 bp	0.5	52.2
ycf39_p3	GAATTTTCGATAGCCGGGGT	CAAGGTCACGACGATTAGGA	50.0	50.0	58.0	57.1	1677 bp	0.5	52.2
ycf39_p4	GAATTTTCGATAGCCGGGGT	TCAAGGTCACGACGATTAGG	50.0	50.0	58.0	57.1	1678 bp	0.5	52.2
ycf39_p5	AATTTTCGATAGCCGGGGTC	CGATTAGGATTTCGTGACGGA	50.0	47.6	58.0	57.9	1665 bp	0.5	51.1