Hotspot

sufC

Genome · 12566-13321 · 756 bp

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Pi 0.116

MarkerSeek score 79.40

Primer available yes

Label sufC

Feature Metadata

label_name: sufC
feature_type: gene
region: Genome
length_bp: 756
variable_sites: 88
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
sufC_p1	ACTCGAAGTCGCATTGTCTC	GCCTCCGCTAAGAATGTGAA	50.0	50.0	58.0	58.0	1320 bp	0.5	59.7
sufC_p2	CTCGAAGTCGCATTGTCTCA	GCCTCCGCTAAGAATGTGAA	50.0	50.0	58.0	58.0	1319 bp	0.5	59.7
sufC_p3	CTTATGCGCAGGGGATTCTT	GCCTCCGCTAAGAATGTGAA	50.0	50.0	58.0	58.0	1507 bp	0.5	59.6
sufC_p4	TTATGCGCAGGGGATTCTTC	GCCTCCGCTAAGAATGTGAA	50.0	50.0	58.0	58.0	1506 bp	0.5	59.5
sufC_p5	GCGCAGGGGATTCTTCTAAA	GCCTCCGCTAAGAATGTGAA	50.0	50.0	58.0	58.0	1502 bp	0.5	59.5