Hotspot

sufB

Genome · 11109-12566 · 1458 bp

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Pi 0.112

MarkerSeek score 79.30

Primer available yes

Label sufB

Feature Metadata

label_name: sufB
feature_type: gene
region: Genome
length_bp: 1458
variable_sites: 163
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
sufB_p1	TTACTGTGTACGCAGCATCC	CAATTGGATCGACTTCGGGT	50.0	50.0	58.0	58.0	2223 bp	0.5	59.8
sufB_p2	TTACTGTGTACGCAGCATCC	CCAAATGCGAACGTTGTTCA	50.0	45.0	58.0	57.9	2103 bp	0.5	59.1
sufB_p3	CGTTTGGCACTGGATCTACA	CAATTGGATCGACTTCGGGT	50.0	50.0	57.9	58.0	2406 bp	0.5	59.1
sufB_p4	TTTGGCACTGGATCTACACG	CAATTGGATCGACTTCGGGT	50.0	50.0	57.9	58.0	2404 bp	0.5	59.1
sufB_p5	TTACTGTGTACGCAGCATCC	CACCCGAAAACCCTTCGTTA	50.0	50.0	58.0	58.1	2313 bp	0.5	59.0