Hotspot

rps3

Genome · 94169-94816 · 648 bp

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Pi 0.186

MarkerSeek score 80.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 648
variable_sites: 119
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CATTCTGTAGCAGCAAACGC	TTACCACGCATTCTTCCTCG	50.0	50.0	58.1	58.0	970 bp	0.5	59.1
rps3_p2	CTGTAGCAGCAAACGCAAAA	TTACCACGCATTCTTCCTCG	45.0	50.0	57.9	58.0	966 bp	0.5	59.0
rps3_p3	CATTCTGTAGCAGCAAACGC	TAATCGTTCGTCGTGTTGCT	50.0	45.0	58.1	57.9	1074 bp	0.5	58.7
rps3_p4	CTGTAGCAGCAAACGCAAAA	TAATCGTTCGTCGTGTTGCT	45.0	45.0	57.9	57.9	1070 bp	0.5	58.6
rps3_p5	CATTCTGTAGCAGCAAACGC	CTGTCGCAACCCAATAGTCA	50.0	50.0	58.1	57.9	1200 bp	0.5	58.4