Hotspot

rpoA

Genome · 101499-102461 · 963 bp

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Pi 0.102

MarkerSeek score 79.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 963
variable_sites: 98
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCGTCTGGTAGTTCTGGGTT	TCGTTTCCCGACACCAATTT	50.0	45.0	58.0	58.0	1746 bp	0.5	59.7
rpoA_p2	TCGTCTGGTAGTTCTGGGTT	TTTGACCTGTTAAACCGCCA	50.0	45.0	58.0	57.9	1939 bp	0.5	59.5
rpoA_p3	TCGTCTGGTAGTTCTGGGTT	GATCTTGTGCTACATGCGGA	50.0	50.0	58.0	58.1	1657 bp	0.5	59.5
rpoA_p4	ACAATATCTTGGGCGTCGTC	TCGTTTCCCGACACCAATTT	50.0	45.0	58.1	58.0	1761 bp	0.5	59.2
rpoA_p5	ACAATATCTTGGGCGTCGTC	TTTGACCTGTTAAACCGCCA	50.0	45.0	58.1	57.9	1954 bp	0.5	59.1