Hotspot

rpl6

Genome · 97639-98181 · 543 bp

Back to Conticribra

Pi 0.129

MarkerSeek score 80.40

Primer available yes

Label rpl6

Feature Metadata

label_name: rpl6
feature_type: gene
region: Genome
length_bp: 543
variable_sites: 70
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl6_p1	ACACTTACGCCGTAAACGTT	AACAACAACAGCACGGAAAC	45.0	45.0	58.1	57.7	1646 bp	0.5	56.6
rpl6_p2	ACACTTACGCCGTAAACGTT	TGGCCGTTCAAATGTACCTT	45.0	45.0	58.1	57.7	1185 bp	0.5	56.6
rpl6_p3	TGGGGTTGGTGGTGAAATTT	AACAACAACAGCACGGAAAC	45.0	45.0	57.8	57.7	1216 bp	0.5	56.4
rpl6_p4	TTGGGGTTGGTGGTGAAATT	AACAACAACAGCACGGAAAC	45.0	45.0	57.8	57.7	1217 bp	0.5	56.4
rpl6_p5	TGGGGTTGGTGGTGAAATTT	TGGCCGTTCAAATGTACCTT	45.0	45.0	57.8	57.7	755 bp	0.5	56.3