Hotspot

dnaK

Genome · 88331-90178 · 1848 bp

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Pi 0.151

MarkerSeek score 78.30

Primer available yes

Label dnaK

Feature Metadata

label_name: dnaK
feature_type: gene
region: Genome
length_bp: 1848
variable_sites: 279
pi: 0.151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK_p1	GCAATTGTCGCACCATTGAA	GGACCCCTTGCAAAATGGTA	45.0	50.0	57.9	58.1	2892 bp	0.5	58.9
dnaK_p2	CGAAGGTGTAGTTGATCCGG	GGACCCCTTGCAAAATGGTA	55.0	50.0	58.1	58.1	2758 bp	0.5	58.8
dnaK_p3	GCCCGTTGTGAACAATTACG	TAAGTGCTTCATGTGGAGCC	50.0	50.0	58.0	57.9	2585 bp	0.5	58.8
dnaK_p4	GCAATTGTCGCACCATTGAA	TAAGTGCTTCATGTGGAGCC	45.0	50.0	57.9	57.9	2273 bp	0.5	58.6
dnaK_p5	CGAAGGTGTAGTTGATCCGG	TAAGTGCTTCATGTGGAGCC	55.0	50.0	58.1	57.9	2139 bp	0.5	58.5