Hotspot

bas1

Genome · 21223-21858 · 636 bp

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Pi 0.101

MarkerSeek score 80.50

Primer available yes

Label bas1

Feature Metadata

label_name: bas1
feature_type: gene
region: Genome
length_bp: 636
variable_sites: 64
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
bas1_p1	CAAGCAGAAGGTCACAGGTT	TAAGGCTGCTTTTCGGGTTT	50.0	45.0	58.0	58.0	1255 bp	0.5	59.7
bas1_p2	CAAGCAGAAGGTCACAGGTT	TTAAGGCTGCTTTTCGGGTT	50.0	45.0	58.0	58.0	1256 bp	0.5	59.7
bas1_p3	CAAGCAGAAGGTCACAGGTT	TTTAAGGCTGCTTTTCGGGT	50.0	45.0	58.0	58.0	1257 bp	0.5	59.7
bas1_p4	GCAGAAGGTCACAGGTTCAA	TAAGGCTGCTTTTCGGGTTT	50.0	45.0	58.0	58.0	1252 bp	0.5	59.7
bas1_p5	GCAGAAGGTCACAGGTTCAA	TTAAGGCTGCTTTTCGGGTT	50.0	45.0	58.0	58.0	1253 bp	0.5	59.7