Hotspot

trnS-GCU-trnD-GUC

Genome · 50641-50713 · 73 bp

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Pi 0.3

MarkerSeek score 82.30

Primer available yes

Label trnS-GCU-trnD-GUC

Feature Metadata

label_name: trnS-GCU-trnD-GUC
feature_type: igs
region: Genome
length_bp: 73
variable_sites: 21
pi: 0.3
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnD-GUC_p1	AATGGCTGAGTGGTCGAAAG	AGACTCGAACTCGCAACTTC	50.0	50.0	58.2	57.9	217-218 bp	1.0	86.9
trnS-GCU-trnD-GUC_p2	AATGGCTGAGTGGTCGAAAG	GAGACTCGAACTCGCAACTT	50.0	50.0	58.2	57.9	218-219 bp	1.0	86.9
trnS-GCU-trnD-GUC_p3	GAAATGGCTGAGTGGTCGAA	AGACTCGAACTCGCAACTTC	50.0	50.0	58.2	57.9	219-220 bp	1.0	86.8
trnS-GCU-trnD-GUC_p4	GAAATGGCTGAGTGGTCGAA	GAGACTCGAACTCGCAACTT	50.0	50.0	58.2	57.9	220-221 bp	1.0	86.7
trnS-GCU-trnD-GUC_p5	GGAGAAATGGCTGAGTGGTC	AGACTCGAACTCGCAACTTC	55.0	50.0	58.3	57.9	222-223 bp	1.0	86.2