Hotspot

ycf1

SSC · 125476-130791 · 5316 bp

Back to Consolida

Pi 0.00752

MarkerSeek score 31.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5316
variable_sites: 60
pi: 0.00752
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACTCCGGCTTTCCAATCAG	TTGTGGGGATCAGACTCAGA	50.0	50.0	58.2	57.7	263 bp	1.0	77.5
ycf1_p2	AACTCCGGCTTTCCAATCAG	TGTGGGGATCAGACTCAGAC	50.0	55.0	58.2	58.4	262 bp	1.0	76.6
ycf1_p3	AAACTCCGGCTTTCCAATCA	TTGTGGGGATCAGACTCAGA	45.0	50.0	57.7	57.7	264 bp	1.0	76.6
ycf1_p4	AAGAAACTCCGGCTTTCCAA	TTGTGGGGATCAGACTCAGA	45.0	50.0	57.6	57.7	267 bp	1.0	76.0
ycf1_p5	AAACTCCGGCTTTCCAATCA	TGTGGGGATCAGACTCAGAC	45.0	55.0	57.7	58.4	263 bp	1.0	75.7