Hotspot

rpl16

LSC · 82727-84239 · 1513 bp

Back to Consolida

Pi 0.00705
MarkerSeek score 37.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1513
variable_sites
16
pi
0.00705
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AAGGCCCCTTTCGTTTCAC AGGGTTGAATAACAATTCGATTGA 52.6 33.3 58.3 56.9 1575 bp 0.666667 26.7
rpl16_p2 AAGGCCCCTTTCGTTTCAC GGGTTGAATAACAATTCGATTGAC 52.6 37.5 58.3 56.9 1574 bp 0.666667 26.7
rpl16_p3 AAGGCCCCTTTCGTTTCAC AACAATTCGATTGACTCCAT 52.6 35.0 58.3 52.9 1565 bp 0.666667 26.7
rpl16_p4 AAGGCCCCTTTCGTTTCAC AGGGTTGAATAACAATTCGATTGAC 52.6 36.0 58.3 58.1 1575 bp 0.666667 26.7
rpl16_p5 AAGGCCCCTTTCGTTTCAC GGGTTGAATAACAATTCGATTGACT 52.6 36.0 58.3 58.1 1574 bp 0.666667 26.7