Hotspot

ndhB-trnL-CAA

IRa · 145421-146001 · 581 bp

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Pi 0

MarkerSeek score 50.60

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 581
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	622-641 bp	1.0	82.5
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	620-639 bp	1.0	82.5
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	AACCTCCACGCTTTTTAGCA	50.0	45.0	57.9	58.0	653-672 bp	1.0	82.2
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	617-636 bp	1.0	81.0
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	621-640 bp	1.0	80.4