Hotspot

ndhH-rps15

SSC · 124635-124774 · 140 bp

Back to Consolida

Pi 0.019

MarkerSeek score 52.10

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 140
variable_sites: 4
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	TCAACGGCTGCTGGTTTATT	45.0	45.0	58.1	58.1	309-321 bp	1.0	82.8
ndhH-rps15_p2	GAGTCGAAGAACACCATGCA	TCAACGGCTGCTGGTTTATT	50.0	45.0	58.2	58.1	318-330 bp	1.0	81.5
ndhH-rps15_p3	TGAGTCGAAGAACACCATGC	TCAACGGCTGCTGGTTTATT	50.0	45.0	58.2	58.1	319-331 bp	1.0	81.5
ndhH-rps15_p4	AACACCATGCATTGATGGGT	CGGCTGCTGGTTTATTTGTC	45.0	50.0	58.1	57.7	305-317 bp	1.0	81.5
ndhH-rps15_p5	ATGCATTGATGGGTGGTGAG	TCAACGGCTGCTGGTTTATT	50.0	45.0	58.2	58.1	303-315 bp	1.0	81.5