Hotspot

ndhG-ndhI

SSC · 120343-120733 · 391 bp

Back to Consolida

Pi 0.0239

MarkerSeek score 43.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 391
variable_sites: 14
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GAAGTGACTCCGTCTCCAAC	GTCGGTTGCCAATGTCAGTA	55.0	50.0	57.9	58.2	809-819 bp	1.0	79.4
ndhG-ndhI_p2	GCAGCTACAAAGTGGGAGTT	GTCGGTTGCCAATGTCAGTA	50.0	50.0	58.1	58.2	686-696 bp	1.0	79.3
ndhG-ndhI_p3	GAAGTGACTCCGTCTCCAAC	GGCTGCAAGGTATATCGGTC	55.0	55.0	57.9	58.2	1186-1196 bp	1.0	78.8
ndhG-ndhI_p4	GAAGTGACTCCGTCTCCAAC	AGTACGGGCTGCAAGGTATA	55.0	50.0	57.9	58.2	1192-1202 bp	1.0	78.7
ndhG-ndhI_p5	GCAGCTACAAAGTGGGAGTT	GGCTGCAAGGTATATCGGTC	50.0	55.0	58.1	58.2	1063-1073 bp	1.0	78.7