Hotspot

atpB-rbcL

LSC · 56392-57103 · 712 bp

Back to Consolida

Pi 0.00375

MarkerSeek score 55.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 4
pi: 0.00375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CTTTGGGTGCATATTCCGGA	55.0	50.0	58.0	57.9	906-912 bp	1.0	80.1
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	TCTTTGGGTGCATATTCCGG	55.0	50.0	58.0	57.9	907-913 bp	1.0	80.1
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	TTTGGGTGCATATTCCGGAG	55.0	50.0	58.0	57.9	905-911 bp	1.0	80.1
atpB-rbcL_p4	CTTCAAGTGTGGAAACCCCA	CTTTGGGTGCATATTCCGGA	50.0	50.0	57.9	57.9	858-864 bp	1.0	80.0
atpB-rbcL_p5	CTTCAAGTGTGGAAACCCCA	TTTGGGTGCATATTCCGGAG	50.0	50.0	57.9	57.9	857-863 bp	1.0	80.0