Hotspot

trnD-GUC-trnY-GUA

LSC · 31024-31425 · 402 bp

Back to Consolida

Pi 0.0149

MarkerSeek score 37.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 9
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGATCAGGAGCAATCGAAA	CGTGAATCGATGGGTGAAGT	50.0	50.0	58.1	58.0	1414-1444 bp	1.0	81.2
trnD-GUC-trnY-GUA_p2	CGGATCAGGAGCAATCGAAA	CACCCTTTTCTGTCGGACAA	50.0	50.0	58.1	58.0	1379-1409 bp	1.0	81.0
trnD-GUC-trnY-GUA_p3	CGGATCAGGAGCAATCGAAA	GAGCTAAGAGTACGATGGCG	50.0	55.0	58.1	58.0	969-996 bp	1.0	81.0
trnD-GUC-trnY-GUA_p4	GGTGCCGGGACACATATATC	CGTGAATCGATGGGTGAAGT	55.0	50.0	57.9	58.0	1660-1698 bp	1.0	80.8
trnD-GUC-trnY-GUA_p5	GGTATCCTCCATCTGACCCA	CGTGAATCGATGGGTGAAGT	55.0	50.0	57.9	58.0	1820-1856 bp	1.0	80.8