Hotspot

trnH-GUG-psbA

LSC · 143-383 · 241 bp

Back to Consolida

Pi 0.0304

MarkerSeek score 47.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 11
pi: 0.0304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGCTGTTGAAGTTCCAAC	50.0	50.0	57.9	57.9	303-305 bp	1.0	80.8
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TGCTGTTGAAGTTCCAACCA	50.0	45.0	57.9	57.9	301-303 bp	1.0	80.7
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	GCTGCTGTTGAAGTTCCAAC	50.0	50.0	58.2	57.9	304-306 bp	1.0	80.6
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TGCTGTTGAAGTTCCAACCA	50.0	45.0	58.2	57.9	302-304 bp	1.0	80.5
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	GCTGCTGTTGAAGTTCCAAC	50.0	50.0	57.7	57.9	296-298 bp	1.0	79.3