Hotspot

ndhK

LSC · 55630-56307 · 678 bp

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Pi 0

MarkerSeek score 26.90

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	AATTGACCCGCTGCTTAACT	AAACAAATCAGATTGAAACAGT	45.0	27.3	57.8	52.8	745 bp	1.0	40.0
ndhK_p2	AATTGACCCGCTGCTTAACT	AACAAATCAGATTGAAACAGTT	45.0	27.3	57.8	52.8	744 bp	1.0	40.0
ndhK_p3	ATTGACCCGCTGCTTAACTT	AAACAAATCAGATTGAAACAGT	45.0	27.3	57.8	52.8	744 bp	1.0	40.0
ndhK_p4	ATTGACCCGCTGCTTAACTT	AACAAATCAGATTGAAACAGTT	45.0	27.3	57.8	52.8	743 bp	1.0	40.0
ndhK_p5	ATTGACCCGCTGCTTAACTT	AAACAAATCAGATTGAAACAGTT	45.0	26.1	57.8	53.6	744 bp	1.0	40.0