Hotspot

atpF

LSC · 15971-17278 · 1308 bp

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Pi 0

MarkerSeek score 59.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1308
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGGGTTGCCATGAGTCTTTC	GTAGCAAAGAGGTGAGGCAA	50.0	50.0	57.8	57.8	1441-1445 bp	1.0	77.1
atpF_p2	GAAGGGTTGCCATGAGTCTT	GTAGCAAAGAGGTGAGGCAA	50.0	50.0	57.8	57.8	1443-1447 bp	1.0	77.1
atpF_p3	GGGTTGCCATGAGTCTTTCT	GTAGCAAAGAGGTGAGGCAA	50.0	50.0	57.8	57.8	1440-1444 bp	1.0	77.1
atpF_p4	AGGGTTGCCATGAGTCTTTC	GAGGTGAGGCAATACAACGA	50.0	50.0	57.8	57.6	1433-1437 bp	1.0	75.5
atpF_p5	GGGTTGCCATGAGTCTTTCT	GAGGTGAGGCAATACAACGA	50.0	50.0	57.8	57.6	1432-1436 bp	1.0	75.5